Mini-cutting supports low-input clonal propagation of Piptadenia stipulacea under semi-arid conditions
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Abstract
Piptadenia stipulacea is a leguminous tree species native to the Caatinga with potential for ecological restoration in semi-arid environments, but information on its vegetative propagation remains limited. We evaluated sprouting dynamics in a seminal mini-garden and tested whether indole-3-butyric acid (IBA) improved adventitious rooting of mini- cuttings. We treated mini-cuttings with 0, 2,000, 4,000, and 6,000 mg L⁻¹ IBA and evaluated rooting, survival, growth, and biomass production. Sprout production varied over time and increased markedly at 90 days, indicating recovery of ministumps after successive pruning. IBA did not improve rooting, survival, growth, or biomass accumulation. The control treatment showed the highest mean rooting and survival values, while the highest IBA concentration reduced overall morphophysiological performance. Intermediate doses produced balanced responses but did not provide a consistent advantage over the absence of growth regulator. We concluded that P. stipulacea can be efficiently propagated by mini- cutting without exogenous auxin under the tested conditions. This response supports a low-input propagation protocol and may reduce operational costs for seedling production aimed at restoration programs in semi-arid regions.
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References
Alvares, C. A., Stape, J. L., Sentelhas, P. C., de Moraes Gonçalves, J. L., & Sparovek, G. (2013). Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift, 22(6), 711–728. https://doi.org/10.1127/0941-2948/2013/0507
Aradóttir, Á., & Halldórsson, G. (2018). Colonization of woodland species during restoration: Seed or safe site limitation? Restoration Ecology, 26(6), 1040–1048. https://doi.org/10.1111/rec.12645
Araújo, J. K. P., Santos, D. F., Araújo, J. S. O., Silva, F. E., Freire, Á. C., Nicolau, J. P. B., Pacheco, M. V., & Araújo, P. C. D. (2025). Propagule age and adventitious rooting in cuttings of Cordia oncocalyx. Revista Árvore, 49(1), e49263888. https://doi.org/10.53661/1806-9088202549263888
Benedito, C. P., Ribeiro, M. C., Paiva, E., & Medeiros, H. L. S. (2019). Dormancy overcoming and germination test in Piptadenia stipulacea (Benth.) Ducke seeds. Revista Ciência Agronômica, 50(3), 333–340. https://doi.org/10.5935/1806-6690.20190040
Bond, W. J., & Midgley, J. J. (2001). Ecology of sprouting in woody plants: The persistence niche. Trends in Ecology & Evolution, 16(1), 45–51. https://doi.org/10.1016/S0169-5347(00)02033-4
Clarke, P. J., Lawes, M. J., Murphy, B. P., Russell-Smith, J., Nano, C. E. M., Bradstock, R., Enright, N. J., Fontaine, J. B., Gosper, C. R., Radford, I., Midgley, J. J., & Gunton, R. M. (2013). Resprouting as a key functional trait: How buds, protection and resources drive persistence after fire. New Phytologist, 197(1), 19–35. https://doi.org/10.1111/nph.12001
Deloso, B. E., Lindström, A. J., Camacho, F. A., & Marler, T. E. (2020). Adventitious root formation of Zamia. HortScience, 55(9), 1463–1467. https://doi.org/10.21273/HORTSCI15212-20
Dias, P. C., Xavier, A., Oliveira, L. S., Paiva, H. N., & Correia, A. C. G. (2012). Vegetative propagation of Anadenanthera macrocarpa. Revista Árvore, 36(3), 389–399. https://doi.org/10.1590/S0100-67622012000300001
Díaz-Sala, C. (2021). Adventitious root formation in tree species. Plants, 10(3), 486. https://doi.org/10.3390/plants10030486
DRYFLOR. (2016). Plant diversity patterns in neotropical dry forests and their conservation implications. Science, 353(6306), 1383–1387. https://doi.org/10.1126/science.aaf5080
Farias, R. M., Freitas, R. M. O., Nogueira, N. W., & Dombroski, J. L. D. (2013). Overcoming seed dormancy in jurema-branca (Piptadenia stipulacea) seeds. Revista de Ciências Agrárias, 56(2), 160–165. https://doi.org/10.4322/rca.2013.024
Freire, Á. C., Maciel, E. R. F., Silva, F. S., Araújo, J. K. P., Cavalcante, B. P., & Araújo, P. C. D. (2025). Clonal propagation of Mimosa caesalpiniifolia: Effects of phenotype, IBA, and leaf area reduction on rooting and plant propagation. Rhizosphere, 36, 101232. https://doi.org/10.1016/j.rhisph.2025.101232
Hartmann, H. T., Kester, D. E., Davies, F. T., & Geneve, R. L. (2018). Plant propagation: Principles and practices (8th ed.). Prentice Hall.
Kildisheva, O. A., Erickson, T. E., Madsen, M. D., Dixon, K. W., & Merritt, D. J. (2018). Seed germination and dormancy traits of forbs and shrubs important for restoration of North American dryland ecosystems. Plant Biology, 21(3), 458–469. https://doi.org/10.1111/plb.12892
Kumar, P., Chaturvedi, A., & Vaishnav, V. (2022). Relationship between endogenous auxin (indole-3-acetic acid) and adventitious rooting in Dalbergia species of different rooting ability. Journal of Tropical Forest Science, 34(2), 149–159. https://doi.org/10.26525/jtfs2022.34.2.149
Leal, I. R., Tabarelli, M., & Silva, J. M. C. (Eds.). (2003). Ecology and conservation of the Caatinga. Editora Universitária UFPE.
Miles, L., Newton, A. C., DeFries, R. S., Ravilious, C., May, I., Blyth, S., Kapos, V., & Gordon, J. E. (2006). A global overview of the conservation status of tropical dry forests. Journal of Biogeography, 33(3), 491–505. https://doi.org/10.1111/j.1365-2699.2005.01424.x
Murphy, P. G., & Lugo, A. E. (1986). Ecology of tropical dry forest. Annual Review of Ecology and Systematics, 17, 67–88. https://doi.org/10.1146/annurev.es.17.110186.000435
Pausas, J. G., & Keeley, J. E. (2014). Evolutionary ecology of resprouting and seeding in fire-prone ecosystems. New Phytologist, 204(1), 55–65. https://doi.org/10.1111/nph.12921
Ratanapongsai, Y. (2020). Seedling recruitment of native tree species in active restoration forest. Forest and Society, 4(1), 243–255. https://doi.org/10.24259/fs.v4i1.9421
Sampaio, E. V. S. B. (1995). Overview of the Brazilian Caatinga. In S. H. Bullock, H. A. Mooney, & E. Medina (Eds.), Seasonally dry tropical forests (pp. 35–63). Cambridge University Press.
Sena, C. M. de, & Gariglio, M. A. (2008). Sementes florestais: Colheita, beneficiamento e armazenamento (Guias Técnicos, Vol. 2). Ministério do Meio Ambiente.
Siegmund, G.-F., Schlaepfer, D. R., Andrews, C. M., Bennion, L. D., Ferguson, J., Jeffries, M. I., Olwell, P., Pilliod, D. S., Simler-Williamson, A. B., Stears, A. E., Zweng, R., & Bradford, J. B. (2025). Supporting dryland restoration success with applied ecological forecasting of seeding outcomes. Restoration Ecology, 34(2), e70179. https://doi.org/10.1111/rec.70179
Silva, A. K. V. da, Aguiar, T. S., Santos, M. E. C. dos, Araújo, J. K. P. de, Freire, Á. C., Salami, G., & Araujo, P. C. D. (2022). Vegetative propagation of Mimosa caesalpiniifolia by mini-cuttings technique. Revista Árvore, 46, e4631. https://doi.org/10.1590/1806-908820220000031
Soto, M. A. S., Ávalos, J. G., Lazcano, A. H., Ávalos, R. G., León, S. H., & López, N. A. F. (2025). Growth in nursery and plant quality of selected trees of Pinus cembroides Zucc. Revista de Gestão Social e Ambiental - RGSA, 19(3), e011741. https://doi.org/10.24857/rgsa.v19n3-126
Souza, C. S., Araújo, P. C. D., Silva, D. Y. B. O., Nogueira, G. A., & Silva, M. J. N. (2022). Vegetative rescue of Azadirachta indica by cuttings. Revista Árvore, 46, e4632. https://doi.org/10.1590/1806-908820220000032
Souza, C. dos S., Machado, E. L. M., Titon, M., Silveira, G. V. dos S., Botrel, R. T., Paiva Neto, E. E. de, Gonzales, D. E., Silva, M. J. N., & Araújo, P. C. D. (2024). Propagação vegetativa por miniestaquia de Mimosa tenuiflora (Mart.) Benth. Observatório de la Economía Latinoamericana, 22, e4758. https://doi.org/10.55905/oelv22n5-131
Souza, L. S., Diógenes, F. E. G., dos Santos Silveira, G. V., da Silva, C. J., & Araujo, P. C. D. (2023). Mini-cutting as a technique to propagate Tabebuia aurea, an important tree found in tropical dry forests. Australian Journal of Crop Science, 17. https://doi.org/10.21475/ajcs.23.17.08.p3892
Souza, C. dos S., Silveira, G. V. dos S., Holanda, A. C. de, Braz, R. L., Freitas, E. C. S. de, Araujo, P. C. D., & Silva, M. J. N. (2026). Morphoanatomical aspects of adventitious rooting and the establishment of an efficient vegetative propagation protocol for Mimosa ophthalmocentra. New Forests, 57, 29. https://doi.org/10.1007/s11056-026-10174-5
Stuepp, C. A., Wendling, I., Xavier, A., & Zuffellato-Ribas, K. C. (2018). Vegetative propagation and application of clonal forestry in Brazilian native tree species. Pesquisa Agropecuária Brasileira, 53, 985–1002. https://doi.org/10.1590/S0100-204X2018000900002
Wendling, I., Trueman, S. J., & Xavier, A. (2014a). Maturation and related aspects in clonal forestry—Part I: Concepts, regulation and consequences of phase change. New Forests, 45, 449–471. https://doi.org/10.1007/s11056-014-9421-0
Wendling, I., Trueman, S. J., & Xavier, A. (2014b). Maturation and related aspects in clonal forestry—Part II: Reinvigoration, rejuvenation and juvenility maintenance. New Forests, 45, 473–486. https://doi.org/10.1007/s11056-014-9415-y
Xavier, A., Wendling, I., & Silva, R. L. (2013). Clonal silviculture: Principles and techniques. Editora UFV.
Yirdaw, E., Tigabu, M., & Monge, A. (2017). Rehabilitation of degraded dryland ecosystems – review. Silva Fennica, 51(1B), Article 1673. https://doi.org/10.14214/sf.1673
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