ALLELOPATHIC POTENTIAL OF Lupinus albus L., CULTIVARS ON MICELIAL GROWTH in vitro OF Gaeumannomyces graminis var. tritici

Abstract

The aim of this study was to evaluate the allelopathic potential of cultivars of Lupinus albus L. Rumbo, Typ Top and Pecosa on the mycelial growth of the fungus Gaeumannomyces graminis var. tritici using aqueous extracts, considering five concentrations (1, 3, 10, 30 and 90% v/v), compared with a control treatment. The methodology consisted of measuring the radius of mycelial growth from the center of the Petri plates with mixed medium APD and extract, every 24 hours to complete the control plate. The experimental design was completely randomized with three replicates per treatment, each Petri plate was an experimental unit. Data analysis was performed using ANOVA and multiple comparison test of Tukey (p≤0.05). The main results showed a differential allelophatic effect of the radical aqueous extracts, often inhibitory and occasionally enhancing the mycelium growth of G. graminis var. tritici. The radical aqueous extract Typ Top, often had higher inhibitory allelopathic effect on the mycelial growth at concentrations of 10%, 30% and 90% v/v. Concentrations of extracts above 30% v/v, showed similar allelopathic effect on mycelial growth, while concentrations below 3% caused a differential response, with Pecosa showing its higher inhibitory effect at 1% and Typ Top at 3%.