A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats

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Published: Jun 30, 1999
DOI: https://doi.org/10.4067/S0301-732X1999000100015
Keywords:
- fluorescence polarization assay
- goats
- Clostridium perfringens
- epsilon toxin antibodies

Main Article Content

F. A. UZAL
Division of Veterinary Pathobiology, School of Veterinary Sciences, The University of Queensland. Brisbane Qld 4072, Australia.
P. SMITH
Animal Diseases Research Institute, Canadian Food Inspection Agency. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9.
K. NIELSEN
Animal Diseases Research Institute, Canadian Food Inspection Agency. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9.
M. LIN
Animal Diseases Research Institute, Canadian Food Inspection Agency. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9
W. R. KELLY
Division of Veterinary Pathobiology, School of Veterinary Sciences, The University of Queensland. Brisbane Qld 4072, Australia.

Abstract

A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation of ten measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later.

Article Details

How to Cite
UZAL, F. A., SMITH, P., NIELSEN, K., LIN, M., & KELLY, W. R. (1999). A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats. Archivos De Medicina Veterinaria, 31(1), 131–134. https://doi.org/10.4067/S0301-732X1999000100015
Issue
Vol. 31 No. 1 (1999)
Section
COMUNICACIONES
Author Biographies

F. A. UZAL, Division of Veterinary Pathobiology, School of Veterinary Sciences, The University of Queensland. Brisbane Qld 4072, Australia.

PhD.

K. NIELSEN, Animal Diseases Research Institute, Canadian Food Inspection Agency. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9.

PhD.

M. LIN, Animal Diseases Research Institute, Canadian Food Inspection Agency. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9

PhD.

W. R. KELLY, Division of Veterinary Pathobiology, School of Veterinary Sciences, The University of Queensland. Brisbane Qld 4072, Australia.

PhD.