Characterization of fibers from gluteus medius muscle in horses by histochemical and immunohistochemical methods

Main Article Content

A. ISLAS
M. QUEZADA
G. MORA
J. L. LÓPEZ-RIVERO
V. MERINO
H. ROJAS
C. CARRILLO
A. ZEGPIG

Abstract

Biopsies were taken at a depth of 4cm from the Gluteus medius muscle of 16 horses. The animals were 6 years old and had not been trained during 3 months before the study. The samples were analized by mATPase miofibrillar technique to determine the fibrillar composition by NADH-TR technique to determine the oxidative capacity of the fibres and immunohistochemistry to determine the fibre type.


The histochemical study revealed an average fibrillar composition of 14, 39, 30 and 17 % for fibres typed I, IIA, IIX oxidative and IIX no oxidative and the inmunohistochemistry study showed 16, 35, 29 y 20% for fibres type I, IIA, IIX and IIA-IIX.


The statistical analyses showed a good relationship between the histochemical and inmunohistochemical method in the determination of fiber types.


The immunohistochemical method allows a good identification of the fiber in adult horses.

Article Details

How to Cite
ISLAS, A., QUEZADA, M., MORA, G., LÓPEZ-RIVERO, J. L., MERINO, V., ROJAS, H., CARRILLO, C., & ZEGPIG, A. (2001). Characterization of fibers from gluteus medius muscle in horses by histochemical and immunohistochemical methods. Archivos De Medicina Veterinaria, 33(1), 55–62. https://doi.org/10.4067/S0301-732X2001000100006
Section
ARTÍCULOS ORIGINALES
Author Biographies

A. ISLAS, Facultad de Medicina Veterinaria, Universidad de Concepción, Casilla 537, Chillán, Chile.

M.V, MS.

M. QUEZADA, Facultad de Medicina Veterinaria, Universidad de Concepción, Casilla 537, Chillán, Chile.

M. V, Dr. Met.Vet.

G. MORA, Facultad de Medicina Veterinaria, Universidad de Concepción, Casilla 537, Chillán, Chile.

MV, MS.

J. L. LÓPEZ-RIVERO, Facultad de Veterinaria, Universidad de Córdoba, España.

M.V., Dr. Med. Vet.

V. MERINO, Facultad de Medicina Veterinaria, Universidad de Concepción, Casilla 537, Chillán, Chile.

BQ, MS.

H. ROJAS, Facultad de Medicina Veterinaria, Universidad de Concepción, Casilla 537, Chillán, Chile.

MV.