Puppies obtained using artificial insemination with chilled extended semen. First report in Chile

Abstract

Using a couple of Husky Siberian, it is described for the first time in Chile a kind of artificial insemination using cooled canine semen. The semen was obtained by digital manipulation and was diluted with UHT semi-skimmed milk and antibiotics in relation 1:4 and cooled at 5ºC. There inseminations were carried out on the third day of the oestrus which was determined through vaginal cytology, considering the beginning of the oestrus when the superficial cells constituted over the 80% of the total vaginal cells in the vaginal smear. Inseminated was carried out using cooled doses for 24 and 48 hours and with an average concentration of 600 x 10⁶ spermatozoa. The pregnancy diagnosis, through a real time ultrasonography, was done 28 days after the last insemination and the bitch gave birth to 4 normal puppies 61 days after last insemination.