Succesfull vitrification with the Open Pulled Straw method (OPS) of bovine blastocysts produced in vitro: Preliminary results

Abstract

The purpose of this work is to present preliminary produced in vitro using the Open Pulled Straw (OPS) method.
Open Pulled Straws were made by pulling 0.25 ml inner and outer diameter were reduced to half of their original and deposited individually in vitrification solutions 1 and dimethyl – sulfoxide (DMSO), for 1 minute and 20 µl containing the embryo pulled into the straw by capillarity into liquid Nitrogen.
Eighty three blastocysts were recovered after thawing. Fifthy four percent of these were re- expanded or hatched after 24 hours of in vitro culture. After 72 hours of in vitro culture 29% of the blastocysts matured. Even though our results are slightly lower to those reported in the literature, they show that it is possible to successfully vitrify bovine blastocysts produced in vitro using the OPS method.