Evaluation of the tube method for concentrating canine platelets: cellular study

Abstract

The aim of this study was to evaluate a manual method for concentrating canine platelets and consequently produce autologous platelet concentrates (APCs) for clinical and experimental proposals. The APCs were obtained by venous blood collection in tubes with ACD solution and were spun at 191 g for 6 minutes. The cell counts of whole blood and APCs, were statistically different (P < 0.01) between the values for platelets, leukocytes, granulocytes, monocytes, but not for lymphocytes. A strong negative correlation (ρ = -0.702 P = 0.011) between the number of lymphocytes and platelets in the APCs was found. The collection efficiency of platelets was 29.9% and the concentration of platelets was 49.4% higher in APCs when compared to the whole blood samples. The results indicated that this simple centrifugation method allows concentrating canine platelets.