Evaluation of a manual method for producing pure-platelet rich plasma (P-PRP) in rabbits: hematological study

Abstract

The objective of this study was to evaluate a manual method to produce two kinds of pure-platelet rich plasma (P-PRP-A and P-PRP-B) in rabbits. Both P-PRP were obtained by drawing blood from the jugular vein into tubes containing sodium citrate as anticoagulant and, centrifuged at 259 g for 6 minutes. The number of concentrated platelets in the P-PRP-A was 716.14 X 10³/μL and 630 X 10³/μL for P-PRP-B. Cell counts were significantly different (P < 0.05) between whole blood and PRP fractions A and B, for RBC counts (10⁶/μL), WBC (10³/μL), PLT (10³/μL) and PDW (%) but not for MPV (fL). Gender differences were found in P-PRP-A for RBC (10⁶/μL), which was higher in males, and the MPV (fL), which was higher in females. In fraction P-PRP-B a difference was found for MPV (fL), which was higher in females. The PDW (%) was higher in males compared with females. The platelet collection efficiency was 20.76% for P-PRP-A and 18.27% for P-PRP-B. The platelet concentration considering whole blood was 186.92% for P-PRP-A and 164.44% for P-PRP-B. The protocol described in this study represents a simple method to obtain P-PRP in rabbits for experimental purposes and even for therapeutic use.