Influence of age, breed lines and season on boar sperm motility and morphology under tropical conditions
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https://orcid.org/0000-0003-1480-4141
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Sperm morphology assessment is essential for maintaining reproductive efficiency and profitability on farms. In tropical countries and smaller-scale farms, the assessment of sperm morphology of the doses used in artificial insemination is not routinely carried out; therefore, its analysis and standardization are necessary. This study evaluated the influence of age, season, and genetic line on sperm morphology in breeding boars under tropical conditions. The study was conducted on commercial farms in the northern region of Costa Rica from 2019 to 2023, with sampling during both the dry and rainy seasons. Sixty-five boars and 312 ejaculates representing Duroc, Landrace, Pietrain, and Pietrain × Duroc genetic lines were analyzed. Animals were classified by age at collection into three groups according to age at the time of collection: < 24 months (G1, n = 19), 24–48 months (G2, n = 26), and > 48 months (G3, n = 33); some animals were analyzed and classified into different groups. Morphology was assessed by Trumorph® fixation and visual examination using a light microscope at 400× magnification. The percentage of sperm morphological abnormalities varied significantly by year, season, and genetic line (P < 0.05). The Pietrain line showed the highest average abnormality frequency, whereas the rainy season exhibited the greatest proportion of abnormal sperm (P < 0.05). In conclusion, season and genetic line significantly influenced sperm morphology in breeding boars. Standardization and validation of morphological assessment are necessary in semen production centers and commercial pig farms.
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Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Referencias
Banaszewska, D., & Andraszek, K. (2021). Assessment of the morphometry of heads of normal sperm and sperm with the Dag defect in the semen of Duroc boars. Journal of Veterinary Research, 65(2), 239–244. https://doi.org/10.2478/jvetres-2021-0019
Banaszewska, D., & Kondracki, S. (2012). An Assessment of the Breeding Maturity of Insemination Boars Based on Ejaculate Quality Changes. Folia Biologica, 60(3), 151–162. https://doi.org/10.3409/fb60_34.151162
Barquero, V., Soler, C., Sevilla, F., Calderón-Calderón, J., & Valverde, A. (2021). A Bayesian analysis of boar spermatozoa kinematics and head morphometrics and their relationship with litter size fertility variables. Reproduction in Domestic Animals, 56(7), 1024–1033. https://doi.org/10.1111/rda.13946
Brito, L. F. C., da Silva, M. C., & Kolster, K. A. (2025). Standardization of Dog Sperm Morphology Classification. Reproduction in Domestic Animals, 60(2), e70024. https://doi.org/10.1111/rda.70024
Broekhuijse, M. L. W. J., Gaustad, A. H., Bolarin Guillén, A., & Knol, E. F. (2015). Efficient Boar Semen Production and Genetic Contribution: The Impact of Low-Dose Artificial Insemination on Fertility. Reproduction in Domestic Animals, 50(52), 103–109. http://doi.wiley.com/10.1111/rda.12558
Calderón-Calderón, J., Sevilla, F., Roldan, E. R. S., Barquero, V., & Valverde, A. (2022). Influence of fat-soluble vitamin intramuscular supplementation on kinematic and morphometric sperm parameters of boar ejaculates. Frontiers in Veterinary Science, 9, 908763. https://doi.org/10.3389/FVETS.2022.908763
Czubaszek, M., Andraszek, K., & Banaszewska, D. (2020). Influence of the age of the individual on the stability of boar sperm genetic material. Theriogenology, 147, 176–182. https://doi.org/10.1016/j.theriogenology.2019.11.018
Fitzpatrick, J. L., & Lüpold, S. (2014). Sexual selection and the evolution of sperm quality. MHR: Basic Science of Reproductive Medicine, 20(12), 1180–1189. https://doi.org/10.1093/molehr/gau067
Flowers, W. (2015). Factors Affecting the Efficient Production of Boar Sperm. Reproduction in Domestic Animals, 50(Suppl 2), 25–30. https://doi.org/10.1111/rda.12529
Fraser, L., Strzeżek, J., Filipowicz, K., Mogielnicka-Brzozowska, M., & Zasiadczyk, L. (2016). Age and seasonal-dependent variations in the biochemical composition of boar semen. Theriogenology, 86(3), 806–816. https://doi.org/10.1016/j.theriogenology.2016.02.035
Gatimel, N., Moreau, J., Parinaud, J., & Léandri, R. D. (2017). Sperm morphology: assessment, pathophysiology, clinical relevance, and state of the art in 2017. Andrology, 5(5), 845–862. https://doi.org/10.1111/andr.12389
Gonzalez-Castro, R., Porflidt, C., Patton, T., Goins, D., & Herickhoff, L. (2022). Effect of season, genetic line and temperature during transport on sperm motility of commercial insemination doses of pooled boar semen: A retrospective study. Reproduction in Domestic Animals, 57(11), 1363–1374. https://doi.org/10.1111/rda.14214
Górski, K., Kondracki, S., & Wysokińska, A. (2017). Ejaculate traits and sperm morphology depending on ejaculate volume in Duroc boars. Journal of Veterinary Research (Poland), 61(1), 121–125. https://doi.org/10.1515/jvetres-2017-0015
Górski, K., Kondracki, S., Wysokińska, A., & Iwanina, M. (2018). Dependence of sperm morphology and ejaculate characteristics on sperm concentration in the ejaculates of Hypor boars. Journal of Veterinary Research, 62(3), 353–357. https://doi.org/10.2478/jvetres-2018-0050
Hackerova, L., Pilsova, A., Pilsova, Z., Zelenkova, N., Tymich Hegrova, P., Klusackova, B., Chmelikova, E., Sedmikova, M., Simonik, O., & Postlerova, P. (2025). Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges. Animals, 15(3), 305. https://doi.org/10.3390/ANI15030305
Hao, M., Zhai, R., Wang, Y., Ru, C., & Yang, B. (2025). A Stained-Free Sperm Morphology Measurement Method Based on Multi-Target Instance Parsing and Measurement Accuracy Enhancement. Sensors, 25(3), 592. https://doi.org/10.3390/S25030592
Hook, K. A., & Fisher, H. S. (2020). Methodological considerations for examining the relationship between sperm morphology and motility. Molecular Reproduction and Development, 87(6), 633–649. https://doi.org/10.1002/mrd.23346
Kamanova, V., Nevrkla, P., Hadas, Z., Lujka, J., & Filipcik, R. (2021). Changes of sperm morphology in Duroc, Landrace and Large White boars depending on the ambient temperature during the year. Veterinární Medicína, 66(5), 189–196. https://doi.org/10.17221/203/2020-VETMED
Keller, A., Maus, M., Keller, E., & Kerns, K. (2024). Deep learning classification method for boar sperm morphology analysis. Andrology 2024, 1-11. https://doi.org/10.1111/andr.13758
Kemoi, V. C., Kashoma, I. P., & Kichuki, M. (2025). Evaluation of The Efficacy of Three Extenders on Boar Semen Quality after Liquid Storage. Journal of Applied Veterinary Sciences, 10(1), 64–73. https://doi.org/10.21608/JAVS.2024.331894.1459
Knecht, D., Jankowska-Mąkosa, A., & Duziński, K. (2017). The effect of age, interval collection and season on selected semen parameters and prediction of AI boars productivity. Livestock Science, 201, 13–21. https://doi.org/10.1016/j.livsci.2017.04.013
Knox, R. V. (2024). Swine fertility in a changing climate. Animal Reproduction Science, 269, 107537. https://doi.org/10.1016/J.ANIREPROSCI.2024.107537
Kondracki, S., Górski, K., & Iwanina, M. (2020). Impact of sperm concentration on sperm morphology of large white and landrace boars. Livestock Science, 241, 104214. https://doi.org/10.1016/j.livsci.2020.104214
Kondracki, S., Iwanina, M., Wysokińska, A., & Huszno, M. (2012). Comparative analysis of Duroc and Pietrain boar sperm morphology. Acta Veterinaria Brno, 81(2), 195–199. https://doi.org/10.2754/avb201281020195
Li, J., Zhao, W., Zhu, J., Wang, S., Ju, H., Chen, S., Basioura, A., Ferreira-Dias, G., & Liu, Z. (2023). Temperature Elevation during Semen Delivery Deteriorates Boar Sperm Quality by Promoting Apoptosis. Animals, 13(20), 3203. https://doi.org/10.3390/ani13203203
Lopez-Rodriguez, A., Soom, A. Van, Arsenakis, I., & Maes, D. (2017). Boar management and semen handling factors affect the quality of boar extended semen. Porcine Health Management, 3, 15. https://doi.org/10.1186/s40813-017-0062-5
Rocha, V. P., Santos Araújo, L. R., Antônio, L., Miranda, M., Mano E Silva, B., Campos Da Silva, Ê., Patrocínio, C., & Santos, D. (2021). Avaliação morfológica de espermatozoides suínos em uma central de inseminação artificial. Revista Brasileira de Higiene e Sanidade Animal, 15(2), 1-12. Retrieved from http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/635
Schulze, M., Mohammadpour, F., Schröter, F., Jakop, U., Hönicke, H., Hasenfuss, T., Henne, H., Schön, J., & Müller, K. (2021). Suitability of semen stress tests for predicting fertilizing capacity of boar ejaculates. Theriogenology, 176, 73–81. https://doi.org/10.1016/j.theriogenology.2021.09.024
Sevilla, F., Murillo, L., Araya-Zúñiga, I., Silvestre, M. A., Saborío-Montero, A., Vargas-Leitón, B., & Valverde, A. (2025). Effects of age, season, breed, and sperm counting chamber on boar semen quality variables in tropical conditions. Austral Journal of Veterinary Sciences, 57, e5701. https://doi.org/10.4206/ajvs.57.01
Sharma, R., Agarwal, A., Rohra, V. K., Assidi, M., Abu-Elmagd, M., & Turki, R. F. (2015). Effects of increased paternal age on sperm quality, re¬productive outcome and associated epigenetic risks to offspring. Re¬productive Biology and Endocrinology, 13(1), 35. https://doi.org/10.1186/s12958-015-0028-x
Soler, C., Cooper, T., Valverde, A., & Yániz, J. (2016). Afterword to Sperm morphometrics today and tomorrow special issue in Asian Journal of Andrology. Asian Journal of Andrology, 18(6), 895–897. https://doi.org/10.4103/1008-682X.188451
Soler, C., García-Molina, A., Contell, J., Silvestre, M., & Sancho, M. (2015). The Trumorph℗® system: The new universal technique for the ob¬servation and analysis of the morphology of living sperm. Animal Reproduction Science, 158, 1–10. https://doi.org/10.1016/J.ANIREPROS¬CI.2015.04.001
Suárez-Mesa, R., Estany, J., & Rondón-Barragán, I. (2021). Semen quality of Colombian Creole as compared to commercial pig breeds. Tropical Animal Health and Production, 53(1), 129. https://doi.org/10.1007/s11250-021-02557-x
Suzuki, T., Shibahara, H., Tsunoda, H., Hirano, Y., Taneichi, A., Obara, H., Takamizawa, S., & Sato, I. (2002). Comparison of the Sperm Quality Analyzer iic variables with the computer-aided sperm analysis estimates. International Journal of Andrology, 25(1), 49–54. https://doi.org/10.1046/J.1365-2605.2002.00324.X
Szablicka, D., Wysokińska, A., Pawlak, A., & Roman, K. (2022). Morphometry of Boar Spermatozoa in Semen Stored at 17 °C—The Influence of the Staining Technique. Animals, 12(15), 1888. https://doi.org/10.3390/ani12151888
Toner, J. P., Mossad, H., Grow, D. R., Morshedi, M., Swanson, R. J., & Oehninger, S. (1995). Value of sperm morphology assessed by strict criteria for prediction of the outcome of artificial (intrauterine) insemination. Andrologia, 27(3), 143–148. https://doi.org/10.1111/J.1439-0272.1995.TB01085.X
Valverde, A., Barquero, V., & Soler, C. (2020). The application of computer-assisted semen analysis (CASA) technology to optimise semen evaluation. A review. Journal of Animal and Feed Sciences, 29(3), 189–198. https://doi.org/10.22358/JAFS/127691/2020
Wysokińska, A., & Kondracki, S. (2019). Heterosis for morphometric characteristics of sperm cells from Duroc x Pietrain crossbred boars. Animal Reproduction Science, 211, 106217. https://doi.org/10.1016/j.anireprosci.2019.106217
Wysokińska, A., & Szablicka, D. (2021). Integrity of Sperm Cell Membrane in the Semen of Crossbred and Purebred Boars during Storage at 17 °C: Heterosis Effects. Animals, 11(12), 3373. https://doi.org/10.3390/ANI11123373
Zhao, Y., Gao, N., Cheng, J., El-Ashram, S., Zhu, L., Zhang, C., & Li, Z. (2019). Genetic Parameter Estimation and Genomic Prediction of Duroc Boars’ Sperm Morphology Abnormalities. Animals, 9(10), 710. https://doi.org/10.3390/ani9100710