Induction of indirect caulogenesis in Eucalyptus globulus

Abstract

Caulogenic induction was studied using mature seeds as initial explants. Four concentrations of BAP (0, 3, 5 and 7 mg L–1) in the mediums MS, B5 and SP were evaluated for the callus formation. The callus formed in the previous stage was used for the caulogenesis, evaluating the effect of two concentrations of ANA (0.1 and 0.5 mg L–1), maintaining constant the BAP (1.0 mg L–1) and the nutritious media from which they came. In the callus formation, the best results were obtained with 7 mg L–1 of BAP (73%) when using the medium B5; differing significantly from MS. For the caulogenesis, the medium SP produced the highest values in formation of shoots (78%) differing significantly from the media B5 and MS. BAP-ANA’s combination (1.0-0.1 mg L–1) was more appropriate for the formation of shoots (62%), although without statistically differing from the other combination. In conclusion, the callus formation is directly related with the concentration of BAP and the nutritious media used. Also, the concentration of BAP from which the callus and the combinations of BAP and ANA come, for the caulogenesis, do not influence significantly in the formation of adventitious shoots, contrary to the used nutritious media.