Potential of organogenesis as a strategy to the in vitro propagation of Persea lingue in the south-central region of Chile

Abstract

Lingue (Persea lingue) is a native arboreal species of Chile, adequate for producing high quality wood and also recognized for its beauty. The effect of supplemented hormonal components in the culture medium of the Persea lingue were studied, morphogenetic capacity and the in vitro regeneration process via histological studies were analyzed. The Murashige and Skoog (MS) basic culture medium was used and complemented with different concentrations of indole 3–butyric acid (IBA) and 6– Benzylaminopurine (BAP) using apical sections of microshoots obtained from in vitro germinated mature embryos. A complete random block design was applied with five repetitions and factorial arrangement of treatments, the experimental unit was a culture recipient containing one explant. The variance analysis showed statistically significant differences (P = 0.001) of the treatment factor on the above response, variable number of buds per explant and caulinary elongation. Regarding Duncan’s multiple range test, it was possible to confirm that the treatments with significant differences were 0.1 mg L^–1 of IBA and 2.0 mg L^–1 of BAP for the induction of buds per explant and 0.05 mg L^–1 of IBA and 0.5 mg L^–1 of BAP for caulinary elongation. The histological analyses revealed that the proliferation of meristematic cells was originated in the epidermic and subepidermic tissue. As well, the study confirmed in vitro regeneration process through direct organogenesis. These results ratify that organogenesis can be a potential biotechnological tool, useful for massive rescue, conservation, and propagation of native species under a critical status.